Because of their superb metabolic plasticity and stress resistance, eukaryotic microbes such as oleaginous yeasts are extensively used for the conversion of complex feedstocks. Our efforts are devoted to develop tools, platform strains and eventually advanced cell factories for lipid products. Key topics include:
1) Engineering of oleaginous yeasts
2) Creation of synthetic biosystems
Articles:
1. Zhang Y,
Zhang SF, Chu YD, Zhang Q, Zhou RH, Yu D, Wang S, Lyu LT, Xu GW, Zhao
ZK*. “Genetic manipulation of the interconversion between diacylglycerols
and triacylglycerols in Rhodosporidium toruloides” Front.
Bioeng. Biotechnol. 2022, 10, 1034972.
2. Lyu LT*,
Chu YD, Zhang SF, Zhang Y, Huang QT, Wang S, Zhao ZK*. “Engineering the
oleaginous yeast Rhodosporidium
toruloides for improved resistance against inhibitors in biomass
hydrolysates” Front. Bioeng. Biotechnol.
2021, 9, 768934.
3. Jiao X,
Zhang Y, Liu XJ, Zhang Q, Zhang SF*, Zhao ZK*. “Developing a CRISPR/Cas9 system
for genome editing in the basidiomycetous yeast Rhodosporidium toruloides” Biotechnol.
J. 2019, 14, 1900036.
4. Wang YN,
Zhang SF*, Zhu ZW, Shen HW, Lin XP, Jin X, Jiao X, Zhao ZK*. “Systems analysis
of phosphate-limitation induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides” Biotechnol. Biofuels 2018, 11, 148.
5. Zhu ZW,
Zhou YJ, Krivoruchko A, Grininger M, Zhao ZK*, Nielsen J*. “Expanding the
product ortfolio of fungal type I fatty acid synthases” Nat. Chem. Biol. 2017, 13(4),
360–362. (Cover Article)
6. Guo J, Zhou
YJ, Hillwig ML, Shen Y, Yang L, Wang YJ, Zhang XN, Liu WJ, Peters RJ*, Chen XY,
Zhao ZK*, Huang LQ*. “CYP76AH1 catalyzes turnover of miltiradiene in
tanshinones biosynthesis and enables heterologous production of ferruginol in
yeasts” Proc. Natl. Acad. Sci. USA 2013, 110(29), 12108–12113.
7. Zhu ZW,
Zhang SF, Liu HW, Shen HW, Lin XP, Yang F, Zhou YJ, Jin GJ, Ye ML, Zou HF*,
Zhao ZK*. “A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides” Nat.
Commun. 2012, 3, 1112. Data
8. Zhou YJ,
Gao W, Rong QX, Jin GJ, Chu HY, Liu WJ, Yang W, Zhu ZW, Li GH, Zhu GF, Huang
LQ*, Zhao ZK*. “Modular pathway engineering of diterpenoid synthases and the
mevalonic acid pathway for miltiradiene production” J. Am. Chem. Soc. 2012,
134(6), 3234–3241.
