个人简历：

        2006.09-2010.60    大连大学  生物工程  工学学士学位
　　 2010.07-2018.05    大连化学物理研究所  生物化工  工学博士学位
　　 2018.06-2021.06    大连化物所生物技术部1816组  助理研究员
        2021.07-                 大连化物所生物技术部1816组  副研究员

所获荣誉：

　　2014-2015学年度，中科院“三好学生”荣誉称号。 
　　2013年，大连化学物理研究所“优秀共青团员”。

主要科研项目：

1. 国家重点研发计划子课题：创制人工辅酶合成途径（2022YFA0912002），2022.11-2027.10，80 万，在研，负责人。

2. 国家自然科学基金青年基金：人工辅酶依赖型1-脱氧木酮糖-5-磷酸还原异构酶创制研究（21907092），2020.01-2022.12，31.2万，结题，负责人。

3. 中科院大连化物所创新研究基金青年基金：NCD介导甲酸驱动甘油转化合成1,3 -丙二醇 （DICP I202020），2020.08-2022.07，100万，结题，负责人。

代表性论文：

1. Li Q, Guo XJ, Wang XY, Wang JT, Wan L, Xue HZ, Zhao ZK*. “Creation of cytochrome P450 catalysis depending on a non-natural cofactor for fatty acid hydroxylation” J. Energy Chem. 2023, 79, 31–36.

2. Liu YX*, Li ZY, Guo XJ, Wang XY, Zhao ZK. “A phosphite-based screening platform for identification of enzymes favoring nonnatural cofactors” Sci. Rep. 2022, 12, 12484.

3. Wan L, Wang XY*, Hu YH, Li Q, Zhao ZK. “Gram-scale biocatalytic  preparation of the non-natural cofactor nicotinamide cytosine dinucleotide” Tetrahedron Lett. 2022,  88, 153368.

4. 梁世玉，万里，郭潇佳，王雪颖，吕力婷，胡英菡，赵宗保.* “构建可合成非天然辅酶的圆红冬孢酵母工程菌”. 中国生物工程杂志 2022, 42(5), 58–68.

5. Wang XY, Feng YB, Guo XJ, Wang Q, Ning SY, Li Q, Wang JT, Wang L, Zhao ZK*. “Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide” Nat. Commun. 2021, 12(1), 2116.
   

6. Guo XJ, Liu YX, Wang Q, Wang XY, Li Q, Liu WJ, Zhao ZK*. “Non-natural cofactor and formate-driven reductive carboxylation of pyruvate” Angew. Chem. Int. Ed. 2020, 59(8), 3143–3146.

7. Guo XJ, Wang XY, Liu YX, Li Q, Wang JT, Liu WJ*, Zhao ZK*. “Structure-guided design of formate dehydrogenase for regeneration of a non-natural redox cofactor” Chem. Eur. J. 2020, 26(70), 16611–16615.

8. Guo XJ, Feng YB, Wang XY, Liu YX, Liu WJ, Li Q, Wang JT, Xue S, Zhao ZK*. “Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase” Bioorg. Med. Chem. Lett. 2019, 29(12), 1446–1449.

9. Liu YX, Feng YB, Wang L, Guo XJ, Liu WJ, Li Q, Wang XY, Xue S*, Zhao ZK*. “Structural insights into phosphite dehydrogenase variants favoring a non-natural redox cofactor” ACS Catal. 2019, 9, 1883–1887.

10. Guo XJ, Shen HW, Liu YX, Wang Q, Wang XY, Peng C, Liu WJ, Zhao ZK*. “Enabling heterologous synthesis of lupulones in the yeast Saccharomyces cerevisiae” Appl. Biochem. Biotechnol. 2019, 188, 787–797.

11. Wang XY, Wang L, Lin XP, Yang XB, Liu WJ, Zhao ZK*. “Visualizing soluble protein mutants by using monomeric red fluorescent protein as a reporter for directed evolution.”Appl Biochem Biotechnol. 2018, 185(1), 81-90.

12. Sun WY, Yang XB, Wang XY, Jiao X, Zhang SF, Luan YS, Zhao ZK*. “Developing a flippase-mediated maker recycling protocol for the oleaginous yeastRhodosporidium toruloides.” Biotechnol. Lett. 2018, 40(6), 993-940.

13. Wang XY, Zhou YJ, Wang L, Liu WJ, Peng C, Zhao ZK*. “EngineeringEscherichia colinicotinic acid mononucleotide adenylyltransferase for fully active amidated NAD biosynthesis.” Appl Environ Microbiol. 2017, 81(13), e00692-17.

14. Sun WY, Yang XB, Wang XY, Lin XP, Wang YN, Zhang SF, Luan YS, Zhao ZK*. “Homologous gene targeting of a carotenoids biosynthetic gene inRhodosporidium toruloidesby Agrobacterium mediated transformation” Biotechnol. Lett. 2017, 39(7), 1001-1007.

15. Wang L, Ji DB, Liu YX, Wang Q, Wang XY, Zhou YJ, Zhang YX, Liu WJ*, Zhao ZK*. “Synthetic cofactor-linked metabolic circuits for selective energy transfer” ACS Catal. 2017, 7(3), 1977–1983.

16. Zhang YX, Liu WJ, Sohail M, Wang XY, Liu YX, Zhao ZK*. “Enzymatic allylation of catechols” Chem. Lett. 2015, 44(7), 949–951.

17. 王磊，刘武军，刘玉雪，王雪颖，赵宗保*. “正交氧化还原体系及其应用” 中国科学：生命科学 2015, 45(10), 969–975.

18. Lin XP, Wang YN, Zhang SF, Zhu ZW, Zhou YJ, Yang F, Sun WY, Wang XY, Zhao ZK*. “Functional integration of multiple genes into the genome of the oleaginous yeastRhodosporidium toruloides” FEMS Yeast Res. 2014, 14(6), 547–555.